Absence of complement component 3 does not prevent classical pathway–mediated hemolysis

Mechanism of Hemolysis by Complement

1. Sensitization confers upon the red cell the property of adsorbing complement from solution. The submicroscopic film of immune serum protein deposited upon the cell surface during sensitization, and completely analogous to the precipitate formed in a soluble antigen-antibody reaction (e.g., sheep serum vs. rabbit anti-sheep serum) acts as absorbent, the degree of sensitization (size of the fi...

Genetic Deficiency of Complement Component 3 Does Not Alter Disease Progression in a Mouse Model of Huntington's Disease.

Several genes and proteins of the complement cascade are present at elevated levels in brains of patients with Huntington's disease (HD). The complement cascade is well characterized as an effector arm of the immune system, and in the brain it is important for developmental synapse elimination. We hypothesized that increased levels of complement in HD brains contributes to disease progression, ...

Overexpression of complement inhibitor Crry does not prevent cryoglobulin-associated membranoproliferative glomerulonephritis.

BACKGROUND Mice overexpressing thymic stromal lymphopoietin (TSLP) develop mixed cryoglobulinemia with renal disease closely resembling human cryoglobulin-associated membranoproliferative glomerulonephritis (MPGN), including glomerular deposits of immunoglobulins and complement. We assessed the effect of complement inhibition through overexpression of Crry (complement receptor-1 related gene/pr...

Absence of Dystrophin Does Not Affect Myogenesis

Duchenne muscular dystrophy (DMD) is caused by the absence of the protein dystrophin in the muscle cells. The function of dystrophin is still not clear. For enabling study of the molecular function of dystrophin, we used small inhibitory RNA (siRNA) for suppressing the expression of the protein in two muscle cell lines and achieved a quantitative knockdown. We applied two-dimensional differenti...

Ig Allotypic Inclusion Does Not Prevent B